Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In vitro antioxidant and antimicrobial activities of aerial parts of Algerian Jurinea humilis DC (Asteraceae)

Radia Ayad

¹Varenbiomol, Department of Chemistry, University Mentouri Constantine 1, 25000 Constantine, Algeria; ²Department of Biotechnology and Molecular Biology, Faculty of Science and Arts, Aksaray University, Aksaray, Turkey.

For correspondence:- Email: radia.ayad@yahoo.fr Tel:+21331811102

Accepted: 22 November 2017 Published: 29 December 2017

Citation: Ayad R. In vitro antioxidant and antimicrobial activities of aerial parts of Algerian Jurinea humilis DC (Asteraceae). Trop J Pharm Res 2017; 16(12):2903-2909 doi: 10.4314/tjpr.v16i12.14

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To assess the polyphenolic composition of various solvent extracts of Jurinea humilis DC. as well as their in vitro biological effects.

Methods: The crude extracts of the aerial parts of Jurinea humilis were obtained by maceration method with dichloromethane (DCM), methanol (MeOH) and ethyl acetate (EtoAC), separately. Folin-Ciocalteu and aluminum chloride procedures were used to quantify the total phenolic and flavonoid contents, respectively, while antioxidant properties were determined by two methods: phosphomolybdenum and DPPH assays. Antimicrobial activity was also evaluated by disc diffusion method.

Results: The ethyl acetate extract exhibited the highest amount of total phenolic content (TPC, 169.14 ± 7.22 mg gallic acid equivalent/g sample) and total flavoniod contents (TFC, 104.91±0.22 mg rutin equivalent/g sample). The extract (EtoAC) also showed the strongest antioxidant activity of the three extracts, especially DPPH assay (IC₅₀ = 0.16 ± 0.00 mg/mL), as well as demonstrated the highest activity against the pathogenic strains tested.

Conclusion: The results suggest that J. humilis DC. is rich in phenolic and flavonoid contents which may be responsible for good antioxidant and antimicrobial properties. Thus, J. humilis may be a promising source of new antioxidant agents and pharmaceuticals

Keywords: Jurinea humilis, Phenolic, Flavonoid, Antioxidant, Antimicrobial